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Rabbit Anti-JNK1+JNK2+JNK3 antibody (bs-2592R)
~~~Bioss促销~~~
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说明书: 50ul  100ul  200ul
20ul/480.00元
50ul/880.00元
100ul/1580.00元
200ul/2480.00元
大包装/询价

产品编号 bs-2592R
英文称号 JNK1+JNK2+JNK3
中文称号 氨基末端激酶1/2/3抗体
别    名 JNK1 + JNK2 + JNK3; JNK1/2/3; JNK1+2+3; JNK1 + JNK2 + JNK3; MAPK10; c Jun N terminal kinase 1; c Jun N terminal kinase 2; c Jun N terminal kinase 3; JNK; JNK1; JNK2; JNK2ALPHA; JNK2BETA; JNK3; MAPK8; MAPK9; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 9; SAPK(beta); Stress activated protein kinase JNK1; Stress activated protein kinase JNK2; Stress activated protein kinase JNK3; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA.  
Specific References  (1)     |     bs-2592R has been referenced in 1 publications.
[IF=2.42] Li, Mingyong, et al. "c-Jun N-Terminal Kinase is Upregulated in Patients With Hypospadias." Urology 81.1 (2013): 178-183.  IHC-P ;  Human.  
PubMed:23273084
研讨范围 细胞生物  细胞凋亡  糖尿病  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反响 Human, Mouse, Rat, Dog, Pig, Cow, 
产品运用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42-47kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human JNK1/2/3:151-250/384 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保管条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品引见 JNK1(MAPK8) is a member of the MAP kinase family. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7.
JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.
JNK3 is a neuron-specific form of c-Jun N-terminal kinases. Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways of neuronal apoptosis.
The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity.


SWISS:
Q61831

Gene ID:
5599

Database links:

Entrez Gene: 5599 Human

Entrez Gene: 26419 Mouse

Entrez Gene: 116554 Rat

Omim: 601158 Human JNK1

SwissProt: P45983 Human JNK1

Unigene: 138211 Human JNK1

Unigene: 21495 Mouse JNK1

Unigene: 4090 Rat JNK1

Entrez Gene: 5601 Human JNK2



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42-47 kD
Observed band size:42-52 kD
Sample:
Cerebrum(Rat) Lysate at 40 ug
Cerebrum(Mouse) Lysate at 40 ug
Heart(Rat) Lysate at 40 ug
Heart(Mouse) Lysate at 40 ug
Cerebellum(Rat) Lysate at 40 ug
Cerebellum(Mouse) Lysate at 40 ug
Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46'54 kD
Observed band size: 54 kD
Sample:
Hela(Human) CellLysate at 30 ug
Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42-47 kD
Observed band size:42-52 kD
Sample:
K562 (Human) Lysate at 30 ug
Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42-47 kD
Observed band size:42-52 kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-JNK1/2/3 Polyclonal Antibody, Unconjugated(bs-2592R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-JNK1+JNK2+JNK3 antibody (bs-2592R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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